摘要
This study was undertaken to observe consecutively the morphology of testis andepididymis and the changes of enzymatic activities of AKP, ACP,3β-HSD andLDH-C4 in male rats treated with GTW 30 to 80 days.In addition,male antifertility effect and its possible reversibility were also observed.The results showed that GTW is apotential testicular toxicant in the animals.It can cause damage of the sperm cells withclose relation to the treated time and dosage.Sloughing of sperm cells and manymultinucleated giant cells in the seminiferous tubules were found on day 40 and 50 afterthe treatment.The lubules were hyperatrophic and the sperm cells were almost absent atthe end of the study(80days).No obvious morphological alteration was observed in theopididymal epithelial tissue.But changes of quality and number of the spermatozoa inepididymides were found prior to those of lestes.Reduced number and abnormal caudasperm were observed 30 days after the treatment.The ACP activity of Serloli cells increased slightly,whereas foe activities of ACP and LDH-C4 decreased gradually as thetreated lime prolonged.The 3β-HSD Of Leydik.cells was changed or subtle by m-Phaseor treatment and dramatically decreased at the end of treatment.The infertility caused byGTW was reversible 8 weeks after cessation of the treatment.
This study was undertaken to observe consecutively the morphology of testis andepididymis and the changes of enzymatic activities of AKP, ACP,3β-HSD andLDH-C4 in male rats treated with GTW 30 to 80 days.In addition,male antifertility effect and its possible reversibility were also observed.The results showed that GTW is apotential testicular toxicant in the animals.It can cause damage of the sperm cells withclose relation to the treated time and dosage.Sloughing of sperm cells and manymultinucleated giant cells in the seminiferous tubules were found on day 40 and 50 afterthe treatment.The lubules were hyperatrophic and the sperm cells were almost absent atthe end of the study(80days).No obvious morphological alteration was observed in theopididymal epithelial tissue.But changes of quality and number of the spermatozoa inepididymides were found prior to those of lestes.Reduced number and abnormal caudasperm were observed 30 days after the treatment.The ACP activity of Serloli cells increased slightly,whereas foe activities of ACP and LDH-C4 decreased gradually as thetreated lime prolonged.The 3β-HSD Of Leydik.cells was changed or subtle by m-Phaseor treatment and dramatically decreased at the end of treatment.The infertility caused byGTW was reversible 8 weeks after cessation of the treatment.
