非小细胞肺癌肿瘤微血管密度检测的临床意义

Clinical significance of intratumoral microvascular density in human non-small cell lung carcinomas

目的 :研究肿瘤微血管密度与非小细胞肺癌临床病理指标及预后的关系 ,探讨非小细胞肺癌治疗的新策略。方法 :采用超敏过氧化酶免疫组织化学法检测 10 1例非小细胞肺癌手术切除标本中微血管密度和血管内皮生长因子的表达。结果 :10 1例非小细胞肺癌组织标本平均微血管密度计数为 (2 5 9± 9 63 )个 /高倍视野 ;微血管密度与淋巴结转移有关 ,高密度组淋巴结转移阳性率显著高于低密度组 (P <0 0 1) ,而与其它临床病理指标无显著相关性 (P >0 0 5 ) ;微血管密度值与血管内皮生长因子的表达强度呈显著正相关 (P <0 0 0 1) ;微血管高密度组患者生存期明显短于低密度组 (P <0 0 1) ;Cox风险比例模型分析显示肿瘤微血管密度和临床分期可作为判断非小细胞肺癌患者预后的独立指标。结论 :肿瘤微血管密度与非小细胞肺癌的发生、发展和转移密切相关 ,并可作为评价预后的指标。

Objective To investigate the intratumoral microvascular density (MVD) in non-small cell lung carcinomas (NSCLC), and its relation with clinical features and prognosis of NSCLC. Methods The expression of vascular endothelial growth factor (VEGF) and MVD were analyzed in surgical specimens by SP immunohistochemical method. Results he average MVD of 101 patients is 25.9±9.63; There was no significant difference between low MVD and high MVD groups in clinical features but lymph node metastasis; MVD increased with the higher expression of VEGF, P<0.001; Survival analysis showed that the high MVD group was associated with a high risk of death; By Cox proportional hazard model analysis, the MVD were considered an independent predictor for the prognosis. Conclusion Our results suggest that MVD is closely related to the occurrence、development and metastasis of NSCLC, and it can be used as a special method to determine the prognosis of NSCLC.