敛溃散抗溃疡病机制分析和急性毒性试验

Study on mechanism of Liankui Powder against peptic ulcer and its acute toxic test

目的 探讨敛溃散抗溃疡病机制并初步评价其安全性。方法 抗酸作用采用酸碱滴定法 ,抑制胃蛋白酶活性和胃酸分泌作用分别采用Mett玻管法和大鼠插管法 ,镇痛作用采用小鼠扭体法 ,并观察敛溃散对出、凝血时间 ,以及胃肠蠕动和中枢神经系统的影响。结果 1g敛溃散能中和 7.14mmol盐酸 ,显著降低大鼠胃液总酸度和胃蛋白酶活性 ;减少醋酸引起的小鼠扭体次数 ,缩短兔股动脉出血时间而不影响凝血时间 ,延长异戊巴比妥钠对小鼠的催眠时间。对大鼠胃酸分泌和小鼠胃肠蠕动未见明显影响。小鼠对敛溃散的最大耐受倍数为 171倍。结论 临床使用敛溃散是安全的 ,抗酸、抑制胃蛋白酶、局部止血和黏膜保护以及镇痛等作用是其抗溃疡病的主要机制。

OBJECTIVE To investigate the mechanism of Liankui Powder(LKP) against peptic ulcer and to evaluate the safety. METHOD The antsacid action was determined with acid-base titrimetry. The inhibition to activity of pepsin and secretion of gastric acid were investigated by means of Mett's glass tube and intubation in rats respectively. The analgesic action was investigated by body-twisting in mice. The influences of LKP on bleeding time, clotting time, gastrointestinal peristalsis and central nervous system were observed. RESULTS One gram LKP could neutralize 7.14mmol HCl. LKP reduced the total acidity and pepsin activity of gastric juice in rats, and decreased the frequency of body torsion induced by acetic acid in mice. It shortened femoral artery bleeding time instead of cotting time in rabbits, and prolonged the hypnotic duration of sodium amobarbital in mice. LKP didn't influence the secretion of gastric acid in rats and gastrointestinal peristalsis in mice. The tolerable maximum dose multiple was 171 in mice for LKP. CONCLUSION LKP is safe to be used to treat pepsic ulcer with clinical therapeutic dose, and antiacid, pepsin inhibition, local haemostasis, mucosa protection and analgesia could be primary mechanism.