高效液相色谱法同时测定冬凌草中冬凌草甲、乙素的含量

HPLC in quantitative determination of oridonin and ponicidin in the Rabdosia rubescens

目的 研究冬凌草中冬凌草甲、乙素的含量测定。方法 采用高效液相色谱法进行测定。结果 冬凌草甲素进样量在0 .196～ 0 .980 μg之间线性关系良好 ,相关系数r =0 .9999,回收率为 99.2 9% ,测定的RSD =2 .1%。冬凌草乙素进样量在0 .1712～ 0 .85 6 0 μg之间线性关系良好 ,相关系数r=0 .9999,回收率为 98.6 3% ,测定的RSD =1.9%。 结论 本法简便、准确、灵敏 ,重现性好 ,可用于冬凌草及其制剂的质量控制。

OBJECTIVE To develop a method for quantitative determination of oridonin and ponicidin in the Rabdosia rubescens. METHOD HPLC method was selected to determine the content. RESULTS For oridonin the linear range was 0.196～0.980μg with r=0.9999, the average recovery was 99.29% with RSD 2.1%, For ponicidin, the linear range was 0.1712～0.8560μg with r=0.9999, the average recovery was 98.63% with RSD 1.9%. CONCLUSION The method is simple, precise sensitive and reproduciable. It can be used for the quality control of Rabdosia rubescens and pharmaceutics.