反相高效液相色谱法检测人血浆中的劳拉西泮

Determination of lorazepam in human plasma by RP-HPLC

目的 建立检测人血浆中劳拉西泮浓度的高效液相色谱方法。方法 血浆经乙醚萃取浓缩 ,以ZORBAXEclipseXDB C8(15 0mm× 4 .6mm ,5 μm)为色谱柱 ;以乙腈 水 (38.8∶6 1.2 )为流动相 ,流速为 1.0mL·min-1;以紫外 2 30nm为检测波长。结果 劳拉西泮浓度在 5 .0 0～ 15 0 .0 0 μg·L-1内线性关系良好 (r =0 .9993) ;最低检测限为 2 .5 0 μg·L-1;平均回收率为 (99.0 8±6 .86 ) % ,日内、日间RSD分别为 (5 .4 0± 3.32 ) %和 (7.35± 3.13) %。内标物地西泮的绝对回收率为 (78.87± 4 .2 9) % ;峰面积变异为 5 .4 8%。结论 方法简便、快速、准确可靠 ,适用于劳拉西泮血浓度的测定和药代动力学研究。

OBJECTIVE To develop a high performance liquid chromatography method for the determination of lorazepam in human plasma. METHOD Lorazepam was extracted from plasma with diethyl ether. The analytical column was packed with ZORBAX Eclipse XDB-C 8(150mm×4.6mm,5μm). The mobile phase was acetonitrile-water ( 39∶61) and the flow rate was 1.0mL/min. The UV detection wavelength was 230 nm.RESULTS Excellent liner relationship was obtained from the range of 5.00μg·L -1 to 150.00μg·L -1 (r=0.9993) , the limit determination of lorazepam was 2.50μg·L -1 and the average recovery was (99.08± 6.86)%. The intra-day RSD and inter-day RSD were (5.40±3.32)% and (7.35±3.13)% respectively. The absolute recoveries of Diazepam (internal standard) were (78.87±4.29)%, the area RSD were 5.48%. CONCLUSION The method is simple, rapid, accurate and can be used to determine the lorazepam concentration in plasma and for study of pharmacokinetics.