肝炎平抗大鼠肝纤维化酶组织化学的实验研究

An Experimental Study of Liver Enzymohistochemistry on Anti-fibrotic Effect of Gan Yanping in Rat Livers

目的 进一步研究肝炎平在肝纤维化中的保护作用。方法 动物随机分为 5组 :正常组 (n =8) ,依肝炎平作用时间不同分设A、B、C组 (均n =15 ) ,D组为模型组 (n =15 )。肝炎平灌胃 1ml/(10 0g·次 ) ,1次 /d。模型组皮下注射CCl40 3ml/10 0 g体重 ,2次 /周 ,共用 9周。 结果 HE染色及电镜观察显示 :模型组肝脏失去正常结构 ,部分假小叶形成 ,而肝炎平组肝细胞脂肪变性和肝纤维化明显减少。模型组总胶原纤维面积 (84 0 2 3± 81 6 5 )μm2 及面积百分数 (7 0 0± 0 90 ) %明显增加 ,肝炎平组 (B组 )胶原纤维面积 (14 8 73± 4 5 89) μm2 及面积百分数(1 16± 0 33) % ,较模型组明显减少。模型组单胺氧化酶 (MAO)、酸性磷酸酶 (ACP)活性明显增加 ,琥珀酸脱氢酶 (SDH)活性明显下降。肝炎平组较模型组MAO、ACP活性明显下降 ,SDH活性明显增加。结论 肝炎平能减少氧自由基的释放 ,提高肝细胞的新陈代谢及免疫机能状态 ,有抗纤维化作用 ,是一种可应用于临床的安全、有效的药物。

Objective To observe the protective effects of Gan Yanping on CCl 4-induced hepatic fibrosis in rats. Methods The rats were randomly divided into 5 groups; groups A, B, C and D (n=15 in each group), and normal control group (N group, n=8). Gan Yanping was administered via stomach at a dosage of 1 ml/100 g each time, once every day. In the model group, CCl 4 was injected subcutaneously, 0.3 ml/100 g body weight, twice every week for 9 weeks. Results HE staining and electron microscopic examinations revealed that in the model group, the normal hepatic architecture disappeared and pseudo-lobule formation was observed. However, in the Gan Yanping treatment group, hepatic steatosis and fibrosis were less pronounced. In the model group, the total collagen area and area ratio were (840.23±81.65) μm 2 and (7.0±0.9) %, respectively, significantly greater than in the Gan Yanping treatment group (148.73±45.89) μm 2 and (1.16±0.33) %, respectively. The activities of MAO and ACP were elevated and that of SDH decreased in the model group as compared to the N group. The treatment with Gan Yanping abrogated these enzymatic changes. Conclusion Gan Yanping could reduce oxygen-derived free radicals, and enhance the cellular metabolism and immune function, all resulting in an anti-fibrotic effect. Hence, Gan Yanping can protect the liver from fibrosis. It may be a safe and effective preparation for patient with fibrosis.