Gene distribution of cagII in Helicobacter pylori-infected patients of Zhejiang Province

AIM: To determine the prevalence of genotypes of cagⅡ in Helicobacter pylori( H pylon)-infected patients in Zhejiang Province and investigate the relationship between these genotypes and the types of gastroduodenal diseases.METHODS: One hundred and seventy one clinical isolates were collected from 70 chronic superficial gastritis, 31 chronic atrophic gastritis, 41 gastric ulcer, 21 duodenal ulcer, 3 gastric and duodenal ulcer, and 5 gastric adenocarcinoma patients. Polymerase chain reaction assays were performed for analysis of cagT, ORF13 and ORF10 genes in the cagⅡ region.RESULTS: Of 171 Hpyloriisolates from Zhejiang patients,159(93.0%) were positive for all the three loci. One isolate (0.6%) was negative for all the three loci, and 11(6.4%) were partially deleted in cagⅡ. The positive rates of cagT,ORF13 and ORF10 genes were 97.1%, 94.7% and 99.4%,respectively. In the strains isolated from the patients with diseases including chronic superficial gastritis, chronic atrophic gastritis, gastric ulcer and duodenal ulcer, the sitive rates of cagT were 95.7%, 100.0%, 95.1% and 100.0%, respectively. The positive rates of ORF13 were 94.3%, 93.5%, 95.1% and 100.0%, respectively. The sitive rates of ORF10 were 98.6%,100.0%,100.0% and 100.0%, respectively. The three genes were all positive in the three H pylori strains isolated from the patients with both gastric and duodenal ulcer. In the five strains isolated from the patients with gastric adenocarcinoma,only one isolate was negative for ORF13. There were no significant differences of the cagT, ORF13 and ORF10 genes among the different gastroduodenal diseases including chronic superficial gastritis, chronic atrophic gastritis,gastric ulcer, duodenal ulcer, both gastric and duodenal ulcer and gastric adenocarcinoma (χ^2=3.098, P＞0.05 for cagT;χ^2=3.935, P＞0.05 for ORF13 and χ^2=6.328,P＞0.05 for ORF10).CONCLUSION: The cagⅡ is not a uniform and conserved entity. Although the genes in cagⅡ are highly associated with the gastroduodenal diseases, the clinical outcome of Hpyloriinfection is not reliably predicted by the three genes in cagⅡ in patients from Zhejiang Province.

AIM:To determine the prevalence of genotypes of cagIIin Helicobacter pylori ( H pylori)-infected patients in Zhejiang Province and investigate the relationship between these genotypes and the types of gastroduodenal diseases. METHODS:One hundred and seventy one clinical isolates were collected from 70 chronic superfidal gastritis,31 chronic atrophic gastritis,41 gastric ulcer,21 duodenal ulcer,3 gastric and duodenal ulcer,and 5 gastric adenocarcinoma patients.Polymerase chain reaction assays were performed for analysis of cagT,ORF13 and ORF10 genes in the cagII region. RESULTS:Of 171 Hpyloriisolates from Zhejiang patients, 159(93.0%) were positive for all the three loci.One isolate (0.6%) was negative for all the three loci,and 11(6.4%) were partially deleted in cagII.The positive rates of cagT, ORF13 and ORFlOgenes were 97.1%,94.7% and 99.4%, respectively.In the strains isolated from the patients with diseases including chronic superficial gastritis,chronic atrophic gastritis,gastric ulcer and duodenal ulcer,the sitive rates of cagT were 95.7%,100.0%,95.1% and 100.0%,respectively.The positive rates of ORF13 were 94.3%,93.5%,95.1% and 100.0%,respectively.The sitive rates of ORF10 were 98.6%,100.0%,100.0% and 100.0%,respectively.The three genes were all positive in the three H pylori strains isolated from the patients with both gastric and duodenal ulcer.In the five strains isolated from the patients with gastric adenocarcinoma, only one isolate was negative for ORF13.There were no significant differences of the cagT,ORF13and ORFlOgenes among the different gastroduodenal diseases including chronic superficial gastritis,chronic atrophic gastritis, gastric ulcer,duodenal ulcer,both gastric and duodenal ulcer and gastric adenocarcinoma (x^2=3.098,P>0.05 for cagT;x^2=3.935,P>0.05 for ORF13 and x^2=6.328,P>0.05 for ORF10).