淡色库蚊生殖滞育的神经内分泌调节

NEUROENDOCRINE REGULATION OF REPRODUCTIVE DIAPAUSE IN CULEX PIPIENS PALLENS

刚羽化的淡色库蚊(Culex pipiens pallens)去首后,其第1卵泡停滞在N期,不能进一步发育。5天以上日龄滞育蚊去首后,第1卵泡约在去首后24小时即从N期开始发育。组织切片发现,滞育蚊咽侧体(CA)体积小,形态瘦长,略呈圆锥形,胞核着色深,排列紧密,胞质少,似处于失活状态。滞育蚊卵巢转种到发育蚊体内,其卵泡可以发育,而转种到滞育蚊体内则不能发育。类保幼激素(JHA,ZR515)及从发育蚊血淋巴液中提取的粗制保幼激素都能使滞育蚊的卵泡发育。

The primary follicles cannot grow further, and stop at N stage when adults are decapitated soon after emergence in diapausing Culex pipiens pollens. The primary follicles begin ro develop about 24 hours after being decapitated in the 5-day old diapausing adults. Thus the role played by neuroendocrine of the head in regulation of folliaular development is conspicuous. The corpora allata (CA) of diapausing mosquitoes are characterized by their small size, little cytoplasm, no vacuole and being inactive, so there is no indication of juvenile hormone (JH). synthesis and secretion. The primary follicles of diapausing ovary can develop when they are nansplanted into the abdomen of reproductive mosquitoes, but they fail to grow in diapausing mosquitoes. ZR 515 (JHA) and JH extracted from haemolymph of reproductive mosquitoes can trigger diapausing follicles to develop.