黔产毛郁金的镇痛、止血作用实验研究

Study on the Analgesic and Arresting Bleeding Effects of

目的 观察黔产毛郁金根茎的水提取物 ( AQE)和乙醇提取物 ( AL E)的镇痛、止血作用及毒性。方法 采用小鼠扭体法、热板法 ,断尾出血时间 ( BT)、血浆钙时间 ( RT)及 L D50 测定法。结果 AQE和 AL E均能明显抑制小鼠扭体反应及延长热致痛潜伏期。 AQE能明显缩短小鼠 BT及 RT。 AL E和 AQE小鼠口服耐受量达 4 g/ kg和 12 .5 g/ kg 2 4 h ig3次 (相当原生药量 10 0 g/ kg 2 4 h ig 3次 ) ;AQE小鼠 ip L D50 为 7.3±1.1g/ kg(相当原生药生药量 5 8.4 g/ kg)。结论 黔产毛郁金具有镇痛及止血的药理学基础 ,其止血作用与内凝系统的凝血因子有关。且毒性较低。

Objective To observe the analgesic and arresting bleeding effects of the aqueous extract (AQE) and alcoholic extract (ALE) of rhizome of C. aromatica from Guizhou, and to determine the acute toxicity of the extracts. Methods The indexes were tested as following: writhes of abdominal cavity on mice caused by ip of acetic acid, the incubation of pain induced by heated plate stimulation in mice , bleeding time (BT) by cutting the mouse′s tail, recovery time (RT) of decalcifying plasma clotting by adding CaCl2 and LD50.Results AQE and ALE could obviously decrease the numbers of writhe and prolong the incubation of pain of mice. AQE could obviously shorten BT and RT of mice. The tolerable dose of AQE for mice was 12.5 g/kg and that of ALE was 4 g/kg by ig three times in 24 h (equal to 3×100 g/kg in 24 h). LD 50 of AQE was 7.3±1.1 g/kg (equal to 58.4 g/kg of dried herbs) by ip. Conclusion The pharmacological basis of C. aromatica on analgesia and arresting bleeding are proved, and the arresting bleeding effect is realized by affecting intrinsic coagulation system. The acute toxicity of C. aromatica is slight.