伊贝母体细胞胚和器官发生几种大分子活性的比较研究

The Comparative Study of Several Macromolecules Activities Somatic Embryogenesis and Organogenesis of F.Pallidiflora Schrenk

本文报道了伊贝母(Fritillaria pallidiflora Schrenk)组织培养中体细胞胚发生和器官发生过程中的核酸、蛋白质含量及过氧化物酶活性动态的变化.共同特点是,培养初期几种大分子物质的含量均呈显著上升的趋势.说明两种途径均伴随着大分子物质的迅速合成.以后随着形态建成的进程,也都呈现相似的趋势.而 RNA 和蛋白质的合成总是先于 DNA 的合成,说明这几种大分子的动态变化具有顺序性.其区别在于,体细胞胚胎发生过程几种大分子的活性高于器官发生过程,前者是一个更为活跃的生理生化过程.此外,在体细胞胚胎发生途径中,RNA、DNA、蛋白质含量和过氧化物酶活性高峰期分别在培养第3、5、3和5d 时,而器官发生途径分别在第5、10、5和10 d 时.这种不同反映了两种途径在形态建成中的差异,也说明大分子代谢动态总是和形态发生相关.

In the paper,synthetic kinesis of nucleic acid and protein and peroxidase insomatic embryogenesis and organogenesis of F.Pallidiflora schrenk was comparativelystudied.Common features are:in the early stage of culture,the contents of severalmacromolecules markedly rise.This shows that either formation of embryonic cells,ordedifferentiation of organogenesis is always accompanied by rapid synthesis of macro-molecules.Later,they show a similar synthetic tendency in morphogenesis.Besides,RNA and protein synthesis are always in advance of DNA.This shows that the kineticchange of several macromolecules has a certain regularity.Their differences are:Theactivity of several macromolecules in the process of somatic embryogenesis is obviouslyhigher than that in the process of organogenesis.Therefore,the somatic embryogenesisis a more active physiologic and biochemical process.Besides,the firest peak curves ofRNA,DNA,protein and peroxidase in somatic embryogenesis appeared after 3,5,3,and 5 days respectively,but they appeared 5,10,5 and 10 days in organogenesis.Thisdifference reflects the variance of two pothways.It simultaneously illustrates thatmetabolic kinesis of macromolecules is always concerned with morphogenesis.