草莓(Fragaria ananassa Duch)茎段组织培养的研究

Study on tissue culture of the stem of Fragaria ananassa Duch

实验在草莓茎段的组织培养过程中,对外植体不同消毒时间,最佳诱导培养基、分化培养基与生根培养基进行了筛选。结果表明,0.1%升汞对茎段消毒2min时效果最好;诱导愈伤的最佳培养基为:MS+1.5mg·L-16-BA+0.1mg·L-1NAA;最佳分化的培养基为:MS+2.0mg·L-16-BA+0.2mg·L-1NAA;最佳生根培养基为:MS+0.25mg·L-16-BA。

In the process of studies on different sterilize time of explant,the preferable mideia of callus dif - ferentiation and rooting.The result shows that explant sterilized in0.1%aqueous mercuric chloride for2min has the best effect;the preferable medium of callus is MS+1.5mg·L -1 6-BA+0.1mg·L -1 NAA;differentiation is:MS+2.0mg·L -1 6-BA+0.2mg·L -1 NAA;and the preferable medium of rooting is:MS+0.25mg·L -1 6-BA。