血清中痕量铂的示波极谱分析

OSCILLOPOLAROGRAPHIC DETERMINATION OF TRACE PLATINUM IN SERUM

本文采用示波极谱法，提出了在氯化铵－六次甲基四胺－柠檬酸三铵－硫酸底液体系中测定血清中痕量铂的方法。在该体系内样品中铂于滴汞电极上产生一灵敏的催化波，峰电位为－０．９２Ｖ。建立了适宜血清样品的消化条件和最佳底液体系。方法检出限为０．０１ｎｇ·ｍｌ＾－１，加标回收范围为８０．２％～１１７．６％，平均回收率为９７．４％，变异系数为２．５％～１２．９％，线性范围为０．１～１００ｎｇ·ｍｌ。本法灵敏快速，

A methed for determination of trace platinum in serum with oscillographic polarography was reported. In a basesolution of ammonium chloride-hexamethylene tetramine （urotropine）-ammonium citrate-sulphuric acid, platium in the samplecan produce a sensitive polarographic wave at the potential of-0. 92V （vs. SCE）,The digestion condition for serum samplesand the composition of the supporting electrolyte were optimized. The deterction limit was 0. 01 ng·ml-1, The spiked recoverieswere in the range of 80. 2 %～117. 6 % （x=97. 4 %）. The coefficients of variation were 2. 5 %～12. 9 %. The method was sensitive and rapid without the need of expensive equipment. It was succesfully applied to the determina-tion of the serum samples from normal and patients having taken medicine containing cisplatin.