血管内皮生长因子、碱性成纤维细胞生长因子在尖锐湿疣中的表达及其意义

Expression of Vascular Endothelial Growth Factor and Basic Fibroblastic Growth Factor in Condyloma Acuminatum

目的探讨血管内皮生长因子(VEGF)、碱性成纤维细胞生长因子(bFGF)在尖锐湿疣皮损中的表达及其意义。方法链霉亲和素-过氧化物酶法检测VEGF、bFGF在35例尖锐湿疣皮损、10例正常人皮肤组织(包皮)中的表达与分布。结果VEGF、bFGF在尖锐湿疣皮损中的表达水平均明显高于正常人皮肤组织(P<0.001)。尖锐湿疣皮损中VEGF、bFGF阳性细胞遍及表皮全层,但VEGF呈现以基底层、棘层细胞显著表达,到颗粒层表达减弱的模式,而bFGF呈现基底层低表达,从棘层到颗粒层表达逐渐增强,空泡细胞表达最为显著的模式。结论尖锐湿疣皮损中VEGF、bFGF的过量表达在促进真皮微血管生成中可能发挥重要作用。

Objectives To investigate the expression and distribution of two important pro-angiogenic factors, vascular endothelial growth factor (VEGF) and basic fibroblastic growth factor (bFGF), in condyloma acuminatum (CA) and their significance. Methods The expression and distribution of VEGF and bFGF in specimens from 35 cases of CA and 10 normal human skins (foreskin) were detected by streptavidin-peroxidase complex immunohistochemical technique. Results The levels of expression of VEGF and bFGF in CA were both significantly higher than those in normal skins (P﹤0.001). The levels of expression of VEGF and bFGF in CA were both throughout the epidermis but with different characteristic staining patterns, of which VEGF was intense in the basal and spinous layers and faint in the granular layer, while bFGF was faint in the basal layer and gradually became intense from spinous to granular layer and markedly increased in koilocytes. Conclusion The overexpression of VEGF and bFGF may play an important role in promoting new blood vessel formation in the dermis in CA lesions.