反相高效液相色谱法测定雷公藤片中雷公藤甲素含量的改进方法

Improved RP - HPLC method of determination of triptolide quantity in TWH F. tablets

雷公藤(Tripterygium Wifordii Hook.F.)系卫矛科雷公藤属植物,雷公藤片(雷公藤的醋酸乙酯提取物片剂)是目前临床上治疗类风湿关节炎疗效明显的一种药物。目的:建立一种简单、准确的高效液相色谱法测定雷公藤片中雷公藤甲素的含量。方法:经PT-中性氧化铝处理,采用Hypersil C18色谱柱,甲醇-水(45:55)为流动相,流速为1.0ml/min,检测波长为220nm的色谱条件进行测定。结果:在0.5-10μg/ml范围内线性关系良好(R=0.9996),样品分离度均大于1.5,最低检测限为0.1μg/ml,重现性好(RSD为2.278％),日内和日问RSD分别为1.236％和4.285％,平均加样回收率为101.67％。结论:该方法简便、快速、灵敏、重现性好,可用来测定雷公藤片中雷公藤甲素的含量。

Objcet :To develop an improved RP - HPLC method for determining triptolide in the TWH F. Tablets. Methods : The samples were pretreated through PT - neutral aluminum oxide pretreatment column. The determination was carried on RP -HPLC, using Hypersil-C18 column (England) , methanol - water (45:55) as mobile phase with a flow rate of 1.0 ml/ min and at the detection wavelength of 220 nm. Results :The triptolide showed good linearity over the range of 0.5 - 10μg/ml (r = 0. 9996). RSD was 1. 236% and 4. 285% within day and day - to - day, the average recovery was 101. 67%. Conclusion :This method, which is simple, rapid, sensitive, reproducible, is appropriate for the quantity determination of triptolide in the TWH F. tablets.