血红蛋白和胆红素干扰临床化学分析的机理初探

The primary study on mechanisms of interference by hemoglobin and bilirubin in clinical spectrophotometric methods

目的 探讨溶血和黄疸对临床化学分析方法的干扰机制。方法 用紫外 可见分光光度计和全自动生化分析仪观察血红蛋白和胆红素的光谱学行为 ;用NCCLSEP7 P方案评价血红蛋白和胆红素的干扰情况。结果 血红蛋白和胆红素自身在溶液中发生吸光特性的改变 ,双波长不能校正二者所造成的干扰。血红蛋白的光谱学干扰方向与EP7 P结果一致 ,可用血清平行空白校正 ;而胆红素的光谱学干扰方向与EP7 P结果不一致 ,并且不能用平行血清空白校正。结论 血红蛋白和胆红素对临床化学的干扰有不同机制 ,临床生化分析除了正确选择纠正方案外 。

Objective To explore the mechanisms of interference by hemoglobin and bilirubin in clinical colorimetric assays.Methods The spectral behaviors of hemoglobin and bilirubin in solutions were monitored on UV2401PC spectrophotometer and AEROSET 2000 clinical analyzer; Interference studies were conducted using NCCLS protocol EP7-P. Results Hemoglobin and bilirubin had spectral changes in solution and correspondingly given interferences on clinical assays. Bichromatic spectrophotometry could not correct the interferences. Interference by hemoglobin accorded with that being studied with protocol EP7-P and could be corrected with serum blanking, whereas interference by bilirubin did not.Conclusion Hemoglobin and bilirubin interfere clinical colorimetric assays in different mechanisms. Proper methods must be adopted to reduce the interferences of hemoglobin and bilirubin, and it is necessary to offer serum indices for clinic diagnosis.