虾夷扇贝人工诱导雌核发育精子遗传失活及紫外线照射对精子形态结构影响的研究

Studies on the Induction of Artifically Genetic Inactivation and Effects of Ultraviolet Irradiation on the Morphological Structure of Sperm in Japanese Scallop, Patinopecten yessoensis

在 1698μWcm 2 s 1的紫外线照射强度下 ,虾夷扇贝雌核单倍体的最佳诱导时间为 60s。从早期胚胎存活率可以观察到Hertwig效应的存在。随着照射时间增加 ,受精率明显下降 ,而受精卵在到达D形幼虫期之前便停止发育。扫描电镜显示 ,随着紫外线照射强度的增加 ,精子顶体和鞭毛结构趋向于受到更大程度的破坏 ,直到完全失去顶体和鞭毛。并指出这可能是造成受精率降低的主要原因。

Irradiation for 60s at a UV intensity of 1 698μWcm -2 s -1 is the optimum dose to achieve haploid gynogenesis of Japanese scallop, Patinopecten yessoensis. The “Hertwig” effect can be detected on the survival rate of early embryos. The fertilization rate apparently decreases with increasing irradiation time, and the development of the eggs fertilized with the genetically inactivated sperms terminats before reaching the D-shaped larvae stage. Scanning electron microscopy (SEM) is capable of showing the destruction of the sperm acrosome and flagellum in the UV-irradiated sperms. As the duration of UV irradiation increases, the acrosome of sperms tends to suffer greater damage until the sperms eventually lose their flagella. Abnormalities in these structures may account, at least in part, for the decline of the fertilization rate of eggs inseminated with UV-irradiated sperms.