UV-B辐射和久效磷对三角褐指藻DNA共同伤害效应

The Damage Effect of UV-B Radiation and Monocrotophos on DNA of Phaeodactylum tricornutum

运用生态毒理学和生物化学的方法研究了紫外线和久效磷对三角褐指藻DNA的伤害作用。结果表明 ,久效磷对三角褐指藻的生长有抑制作用 ;随着久效磷浓度的增加 ,三角褐指藻DNA损伤程度增加 ;在久效磷浓度固定不变时 ,随着处理时间的延长 ,DNA的损伤程度同样提高 ;在久效磷的处理过程中同时伴有紫外线的辐射处理 ,DNA的损伤程度加剧 ;久效磷处理解除一段时间后 ,DNA损伤程度未明显减轻 ,而UV B处理解除后 ,DNA的损伤可明显恢复。说明DNA的损伤可在一定程度上指示海洋微藻受久效磷伤害的的程度。

The interactive damage process of UV-B radiation and monocrotophos to DNA of Phaeodactylum tricornutum was studied using ecotoxicological and biochemical methods. The results showed that monocrotophos could inhibit the growth and damage DNA of Phaeodactylutum tricornutu, its 48h·EC 50 being 8.04 mg/L. The DNA damage of algal cells increased with increased concentration (from 0 to 5mg/L) and prolonged exposure time (from 0 to 5d) of monocrotophos. The UV-B radiation could enhance DNA damage caused by monocrotophos when Phaeodactylum tricortunum was treated with monocrotophos and 1.4J/m 2 UV-B radiation synchronously. The repair of DNA damage was not remakable after the algae were transferred to a monocrotophos-free fresh medium for 3 days, while DNA damage was obviously decreased after UV-B radiation was removed. All this exhibited that the DNA damage could indicate the damage degree of marine microalgae resulted from monocrotophos exposure.