壳寡糖对番茄叶挥发性抗真菌物质及植保素日齐素的诱导效应

Induction of Antipathogenic Volatiles and Phytoalexin Rishitin in Leaves of Lycopersicon esculentum by Oligochitosan

研究了 0 .3 %壳寡糖诱导番茄 (Lycopersiconesculentum )叶 12 0h后 ,其挥发性物质对番茄枯萎病菌 (Fusariumoxysporum )孢子萌发和菌丝生长的影响 ;采用气相色谱 质谱联用技术 ,检测诱导后番茄叶挥发性物质及植保素日齐素质和量的变化。结果表明 :经壳寡糖诱导后 ,番茄叶挥发性物质对病菌的抑制率较对照组的提高。番茄叶挥发性抗真菌物质总含量为对照组的 1.49倍 ;氧合脂类、萜类及芳香类化合物的含量分别提高了 61% ,10 %和 69%。其中 (E) 2 乙烯醛含量增加了 64 % ,水杨酸甲酯含量增加了 3 8% ,壳寡糖不能诱导番茄植保素日齐素的合成。

In the defensive responses of plants towards external pathogens, volatiles may play a potential role in plant chemical defence strategies. In our work, the leaves of Lycopersicon esculentum were treated with 0.3 % oligochitosan elicitor for 120h. The antipathogenic efficacy of volatiles after oligochitosan treatment was studied by using the method of inverted culture of conidial suspensions. The volatiles and phytolaexin rishitin in the leaves were analyzed by GC/MS. The results demonstrated that the inhibitory effect of volatiles against Fusarium oxysporum was enhanced in oligochitosan-treated leaves. Oligochitosan treatment also increased the amount of total antipathogenic volatiles, which was 1.49 times as compared with that of the control. Phyto-xylipins, terpenoids and phenols were increased by 61%, 10% and 69%, respectively. And there were 1.64-fold and 1.38-fold increases in (E)-2-Hexenal and Methyl Salicylate, respectively. In this system, rishitin was not detectable in oligochitosan-treated leaves.