摘要
提出快速、准确地测定海藻中 3 ( 6 脱氧 6 磺酸基 α D 吡喃型葡萄糖苷基 ) sn 酰基甘油脂 (sulfoquinovosylacylglyc erol,SQAG)含量的方法。该方法是根据天青A能与SQAG形成 1种可溶于氯仿的天蓝色复合物 ,在一定范围内 ,SQAG的量和反应液的颜色强度呈比例关系 ,线性范围为 0 .0 0 44 2～ 0 .114 88μmol,检测限为 0 .0 0 44 2 μmol,该测定方法操作简单、重现性好。经过实验和统计学分析表明 ,海藻中的色素等成分对本方法测定SQAG结果影响较小 ,因此从海藻中提取样品后 ,可以直接采用天青A比色法测定SQAG含量。
A rapid, sensitive spectrophotometeric method for the quantitative determination of 3-(6-deoxy- 6-sulfo-α-D-glucopyranosyl)-sn-acylglycerol (SQAG) is described. The assay method is based upon the formation of a colored complex between cationic dye azure A and anionic SQAG; the complex is extractable by chloroform; the absorbance value of the colored complex is proportional to the amount of SQAG used in the assay in the range of 0.004 42～0.114 88μmol. The method is simple and of good reproducibility, and it is much more sensitive (the lower limit being about 0.004 42μmol) than most methods currently used and has a high degree of precision. The experimental result and statistic analysis proved that the pigments and other compositions can not affect the result of determination. Thus, the method can be applied to samples obtained directly from algae.
出处
《中国海洋大学学报（自然科学版）》
CAS
CSCD
北大核心
2004年第6期1029-1032,共4页
Periodical of Ocean University of China
基金
中国科学院海洋研究所知识创新工程领域前沿项目 (L41 0 2 2 51 0 2 )资助
