维生素C对大鼠油酸型急性呼吸窘迫综合征的保护作用

Protective effect of vitamin C on oleic acid-induced acute respiratory distress syndrome in rats

目的 探讨氧化性肺损伤在急性呼吸窘迫综合征 (ARDS)发病中的作用及维生素C(VitC)的抗氧化保护作用。方法 将 30只大鼠随机分为 3组 :正常对照组、油酸组、VitC组。油酸组、VitC组分别于尾静脉注入油酸、VitC。注射油酸后 2h观察肺系数、肺组织内丙二醛 (MDA)和超氧化物歧化酶 (SOD)的变化 ,以及肺组织病理变化。结果 VitC组比油酸组肺系数、MDA降低 (P <0 .0 5 ) ,SOD活力增加 (P <0 .0 5 ) ,肺组织病理变化明显减轻。结论 氧化性肺损伤参与油酸性ARDS发病 ,VitC具有保护作用。

Objective To study the role of oxidative lung injury in the genesis of oleic acid -induced acute respiratory distress syndrome (ARDS) and the protective effect of vitamin C. Methods Thirty rats were divided into three groups: control, oleic acid, VitC. Oleic acid was injected into the tail vein of rats; VitC was injected into the tail vein before oleic acid injection. Results Two hours after oleic acid injection, it was found in the oleic acid group that the ratio of wet lung-to-body weight and the malondialdehyde (MDA) content in lung were increased(P<0.05), the superoxide dismutase (SOD) decreased(P<0.05), and the histological disruption was more serious, as compared with those in the other two groups. Conclusion Oxidative lung injury leads to oleic acid-induced ARDS; VitC plays a protective role.