中成药中黄连成分的测定

Determination of Coptis Chinesis in Formulary Chinese Medicine

采用了三波长—分光光度法测定中成药—黄连素片中黄连成分的含量,有效地消除了随浓度不同使本底漂移,吸收峰不对称给定量分析造成的影响,并校正了基于干扰组分(添加剂,如淀粉等)的吸收光谱具有线性吸收产生的基线倾斜度,实验结果,回收率为97.82％～101.38％。变异系数小于0.24％(n=9),证明方法的精密度与准确度都令人满意。同时,在微型计算机控制下,操作也简单易行。

The determination of Coptis chinensis in formulary chinese medicine by three wavelength spectrophotometry can eliminate the absorbance error from background changes with concentrations and from asymmetric absorbtion peak problems. The absorbance error of interfering components, sueh as turbid solution and dirty absorbance cells can be removed also. Tbe method is more advantageous thart tranditional spectrophotometry method. The recovery is 97.82％—101.38％ the coefficient of vatiation is 0.24％.