期刊文献+
任意字段
题名或关键词
题名
关键词
文摘
作者
第一作者
机构
刊名
分类号
参考文献
作者简介
基金资助
栏目信息

ABCG2在白血病细胞中的表达及其意义 被引量：3

Analysis of ABCG2 expression on leukemia cells and its significance
下载PDF
导出
摘要 目的探讨三磷酸腺苷(ATP)结合转运蛋白G超家族成员2(ABCG2)在白血病细胞膜上的表达规律及其意义。方法应用CD45/SSC参数设门多色流式细胞术分析61例白血病患者白血病细胞膜ABCG2及其他白血病细胞相关抗原的表达情况。结果61例急慢性白血病患者的白血病细胞ABCG2表达阳性率为36.07%。ABCG2表达阳性率,急性白血病(AL)患者眼34.48%(20/58)演与慢性白血病(CL)患者眼66.67%(2/3)演鸦儿童患者眼26.09%(6/23)演与成人患者眼42.11%(16/38)演差别均无显著性意义(均P>0.05);急性髓细胞性白血病(AML)患者眼46.67%(14/30)演高于急性淋巴细胞性白血病(ALL)患者眼16.67%(4/24)(P<0.05)演;AML患儿眼55.56%(5/9)演也高于ALL患儿眼7.14%(1/14)(P<0.05)演。ABCG2与另一耐药蛋白P糖蛋白(P-gp)及其他细胞表面抗原的表达均无关(r=0.152、-0.075、-0.063、0.026、-0.149,均P>0.05),与髓系标志CD13的表达有关(r=0.307熏P<0.05)。结论ABCG2在AML患儿中的表达较高,其高表达可能导致AML患儿临床耐药。ABCG2和P-gp可能参与了不同的白血病耐药机制。 Objective To investigate the expression of ATP-binding cassette superfamily G member 2 (ABCG2) on leukemia cells and its significance. Methods Blood samples from 61 patients with leukemia were analyzed by CD45/SSC double parameters and multi-color flow cytometry to determine the expression of ABCG2 and other leukocyte differentiation antigens. Results The total positive rate of ABCG2 in 61 patients was 36.07%. ABCG2 expression was not significantly different between acute leukemia (AL) [34.48% (20/58)] and chronic leukemia (CL) [66.67%(2/3)]; ABCG2 expression in children [26.09%(6/23)] was not different from that in adult leukemia [42.11%(16/38)](P>0.05).The positive rate of ABCG2 in AML [46.67%(14/30)]was significantly higher than that in ALL [16.67%(4/24)] (P=0.02). In children AML ABCG2 expression [55.56%(5/9)] was also higher than that in ALL [7.14%(1/14)] (P=0.018). A significant association was found between ABCG2 and a myeloid related antigen CD13 (P=0.018), while no significant correlation of ABCG2 with P-glycoprotein (P-gp) and other leukocyte differentiation antigens was found. Conclusion ABCG2 expression in AML was significantly higher than that in ALL, which suggests ABCG2 may be associated with clinical resistant disease in AML.
作者 张玲燕 汤永民 沈红强 宁铂涛 钱柏芹 石淑文 杨世隆 徐卫群
机构地区 浙江大学医学院附属儿童医院
出处 《浙江医学》 CAS 2004年第11期803-805,853,共4页 Zhejiang Medical Journal
关键词 ABCG2 白血病 基因表达 三磷酸腺苷 多药耐药性 流式细胞术 ABCG2 Leukemia Multi-drug resistance Flow cytometry
分类号 R733.7 [医药卫生—肿瘤]
  • 相关文献

参考文献11

  • 1Doyle LA, Yang W, Abruzzo LV,et al. A multidrug resistance transporter from human MCF-7 breast cancer cells. Proc Natl Acad Sci U S A, 1998 ,95:15665.
  • 2Ross DD, Karp JE, Chen TT,et al. Expression of breast cancer resistance protein in blast cells from patients with acute leukemia. Blood, 2000, 96:365.
  • 3van den Heuvel-Eibrink MM, Wiemer EA, Prins A, et al. In creased expression of the breast cancer resistance protein(BCRP) in relapsed or refractory acute myeloid leukemia (AML).Leukemia, 2002 ,16(5):833.
  • 4van der Kolk DM, Vellenga E, Scheffer GL,et al. Expression and activity of breast cancer resistance protein (BCRP) in de novo and relapsed acute myeloid leukemia. Blood, 2002, 99:3763.
  • 5Steinbach D, Sell W, Voigt A,et al. BCRP gene expression is associated with a poor response to remission induction therapy in childhood acute myeloid leukemia. Leukemia,2002 ,16:1443.
  • 6Abbott BL, Colapietro AM, Barnes Y,et al. Low levels of ABCG2 expression in adult AML blast samples. Blood, 2002,100:4594.
  • 7Krasinskas AM, Wasik MA, Kamoun M, et al. The Usefulness of CD64,other Monocyte Associated Antigens, and CD45 Gating in the Subclassification of Acute Myeloid Leukeamias with Monocytic Differetiation. Am J Clin Pathol, 1998, 110: 797.
  • 8Ejendal KF , Hrycyna CA. Multidrug Resistance and Cancer:The Role of the Human ABC Transporter ABCG2. Curr Protein Pept Sci 2002,3(5):503.
  • 9Bunting KD. ABC transporters as phenotypic markers and functional regulators /of stem cells. Stem Cells, 2002, 20: 11.
  • 10Zhou S, Schuetz JD, Bunting KD, et al. The ABC transporter Bcrp1/ABCG2 is expressed in a wide variety of stem cells and is a molecular determinant of the side-population phenotype. Nat Med, 2001, 7: 1028.

同被引文献36

  • 1房佰俊,宋永平,汪萍,张䶮莉,魏旭东,林全德.K562多药耐药细胞系中肿瘤干细胞样细胞对伊马替尼耐药机制的初步研究[J].白血病．淋巴瘤,2006,15(5):333-337. 被引量：8
  • 2梅家转,周健,郭坤元,魏红梅.低表达MICA/MICB导致NK细胞对人鼻咽癌多药耐药细胞(CNE2/DDP)杀伤活性下降[J].中国肿瘤生物治疗杂志,2006,13(5):349-352. 被引量：8
  • 3Kayo H, Yamazaki H, Nishida H,et al. stem cell properties and the side population cells as a target for interferon a in adult T-cell leukemia/lymphoma. Biochem Biophys Res Commun, 2007, 364 (4) :808-814.
  • 4Zheng X,Seshire A,Ruster B,et al. Arsenic but not all-trans retinoie acid overcomes the aberrant stem cell capacity of PML/ RARα-positive leukemic stem cells. Haematologica, 2007,92 (3) : 323-331.
  • 5Wulf GG,Sadowski B,von Bonin F,et al, Bone marrow side population (SP) cells are donor derived after allogeneic stem cell transplantation in humans. Bone Marrow Transplant, 2005,36 (2): 179-180.
  • 6Dean M,Fojo T,Bates S. Tumour stem cells and drug resistance. Nat Rev Cancer,2005,5(4):275-284.
  • 7Erlichman C, Boerner SA, Hallgren CG, et al. The HER tyrosine kinase inhibitor C11033 enhances cytotoxicity of 7-ethyl-10- hydroxycamptothecin and topotecan by inhibiting breast cancer resistance protein-mediated drug efflux. Cancer Res, 2001,61 (2) : 739-748.
  • 8Nakamura Y, Oka M, Soda H, et al. Gefitinib (" Iressa", ZD1839 )an epidermal growth factor receptor tyrosine kinase inhib itor, reverses breast cancer resistance protein/ABCG2-mediated drug resistance. Cancer Res,2005,65(4) : 1541-1546.
  • 9Houghton PJ,Germain GS, Harwood FC, et al. Imatinib mesylate is a potent inhibitor of the ABCG2 (BCRP) transporter and reverses resistance to topoteean and SN-38 in vitro. Cancer Res, 2004, 64(7) :2333-2337.
  • 10Goodell MA, Brose K, Paradis G, et al. Isolation and functional properties of routine hernatopoietic stem cells that are replicating in vivo. J Exp Med,1996,183(4) :1797-1806.

引证文献3

浙江医学
2004年 第11期

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部