转子莲的繁殖研究

Preliminary Study on Propagation of Clematis patens

对转子莲的繁殖研究表明:转子莲因种子萌发率不高,不宜采用有性繁殖;扦插繁殖采用0.637mmol/L的IAA浸泡2～3min或2.548mmol/L的IAA速蘸对生根有明显促进作用;在转子莲的组培研究中,用叶或茎尖作外植体时,诱导愈伤组织的配方以MS+6-BA2.0mg/L+2,4-D1.0mg/L或MS+6-BA2.0mg/L+IBA1.0mg/L为好;继代增殖的培养过程中以MS+6-BA2.0mg/L+NAA0.5mg/L的配方处理较好;生根培养基配方的筛选以1/2MS+IAA0.8mg/L的培养条件为好.

A research on propagation of Clematis patens was carried out for germplasm conservation. It was showed by the results that generative propagation was not feasible because of the low rate of seed germination. It was proven that the rooting process of cuttage could be promoted by soaking the cuttings in IAA solution with 0.637 m mol/L of concentration for 2 or 3 minutes, or by instant dipping the cuttings into IAA solution with 2.548 m mol/L of concentration prior to the cuttage operation. The result of tissue culture indicated that the formulae of (MS+6-BA (2.0 mg/L)+2,4-D 1.0 mg/L) and (MS+6-BA 2.0 mg/L+IBA 1.0 mg/L) were better at the stage of inducing calli when leaves and apical meristems were adopted as explants. The formula of (MS+6-BA 2.0 mg/L+NAA (0.5 mg/L)) was the best in the process of successive enrichment culture, and the formula of (1/2 MS+IAA 0.8(mg/L)) was the best for root induction.