用单抗夹心ELISA快速检测鸡新城疫病毒

RAPIDLY DETECTING NEWCASTLE DISEASE VIRUS IN VACCINATED CHICKEN FLOCK WITH THE SANDWICH ELISA BASED ON MONOCLONAL ANTIBODY

采用传统的病毒分离的方法将免疫鸡群泄殖腔棉拭样品盲传一代 ,再用新城疫单抗夹心ELISA对F1 尿囊液进行新城疫病毒 (NDV)检测。结果表明本方法具有良好的特异性 ,NDV阳性样品检出率很高 ,且鸡禽流感病毒 (AIV)对此无干扰作用 ;分别检测阳性样品 4 5 3份、阴性样品 2 95 9份 ,与动物世界卫生组织 (OIE)推荐的病毒分离及鉴定、毒力试验的经典检测方法相比较 ,符合率为 99.3%、99.7% ;测定的变异系数为 8% ,稳定性较好 ,同时本法比经典的检测方法缩短检测时间 7d。结果表明用单抗夹心ELISA法检测鸡泄殖腔棉拭样品F1 尿囊液新城疫病毒实用、准确、特异、稳定、快速。

Cloacal swab specimens from vaccinated chicken flo ck were propagated once by SPF embryonated chicken eggs, and then the NDV (Newca stle disease virus) detection of the harvested allantoic fluid was carried out b y the sandwich ELISA. The results showed that this method had good specificity, the detectable rate of positive NDV samples was very high, and the detection was not disturbed by AIV. Detecting 453 positive samples and 2959 negative samples with this method and the traditional diagnostic methods recommended by OIE showe d that the coincidence rate of positive samples was 99.3% and that of negative o nes was 99.7%. The repeatability of detection was quite good and the variati on coefficient was 8%. Compared with the traditional method, this method could s horten the time of detection by 7 days. So using this method to detect NDV is pr actical, accurate, specific and rapid.