小麂线粒体基因组全序列的测定和分析

Sequence and Organization of Muntiacus reevesi mitochondrial Genome

通过建立麂属动物小麂线粒体DNA文库、鸟枪法测序 ,获得了小麂线粒体基因组全序列并对其基因组成、蛋白质的编码序列、tRNA基因等结构作了详细分析 ,这也是国内有关哺乳动物线粒体基因组全序列的首次报道。与其他哺乳动物线粒体基因组全序列的比较研究发现 :全长为 16 35 4bp的小麂线粒体基因组同样编码 13种蛋白质、2种rRNA和 2 2种tRNA ,除了用于调控线粒体DNA复制和转录的D Loop区以外 ,小麂线粒体基因组各基因长度、位置与其他哺乳动物相似 。

A shot-gun DNA sequence strategy was employed,in which the mitochondrial genome library of Muntiacus reevesi has been constructed to obtain the complete mitochondrial genome sequence. The Chinese Muntjac’s mitochondrial genome,consisting of 16354 base pairs which encode genes for 13 proteins,2 rRNAs,and 22 tRNAs,is similar to those mammals in both order and orientation.The sequence of rRNA gene,some of the protein-coding regions and tRNAs are highly homologous in mammals. Differences existing in the length and sequence of the D-loop regions account for the variations in mammals mitochondrial genomes.