摘要
目前已克隆了48个植物抗病(R)基因,其表达产物在不同的物种具有典型的保守结构区域,按其序列的同源性可以将其归为NBS-LRR、eLRR、LRR-STK等几个超家族。根据这些保守序列设计合适的引物进行PCR扩增,即得到抗病基因同源序列(RGA)。对RGA与R基因关系的分析表明,RGA不仅在抗病基因定位和抗病基因系统进化研究中有重要作用,而且有可能为克隆R基因提供一条崭新而又便捷的途径。
Using either map-based cloning or transposon tagging, up to 48 resistance genes (R genes) have been isolated from different plant species. Molecular characterization of these genes uncovered common sequence motifs, even though they confer resistance to a wide spectrum of pathogens. These genes encode proteins that can be grouped into several superfamilies, NBS-LRR, eLRR, LRR-STK and so on, based on the conserved protein domains. PCR was performed with the degenerate primers based on these conserved domains, then the resistance gene analogs (RGA) was obtained. This review tries to analyze the relationships between the RGA and R genes, and summarize the important functions of RGA in mapping R genes and the evolution of R genes. It also believes that RGA can be useful as candidate genes to detect and eventually isolate numerous R genes in agronomically valuable plants.
出处
《分子植物育种》
CAS
CSCD
2004年第6期853-860,共8页
Molecular Plant Breeding
