VEGF和iNOS在喉鳞状细胞癌中的表达及临床意义

The expression of VEGF and iNOS in laryngeal squamous cell carcinoma and its clinical significance

目的 :研究血管内皮生长因子 (VEGF)和诱生型一氧化氮合成酶 (iNOS)在喉鳞状细胞癌组织中的表达及其与临床病理特征之间的关系 ,并分析两者表达的相关性。方法 :采用逆转录聚合酶链反应 (RT PCR)技术 ,检测 4 0例新鲜喉鳞状细胞癌标本和远离肿瘤的正常喉组织中的VEGF和iNOS的mRNA表达。结果 :VEGF和iNOS在肿瘤组织中的阳性表达率明显高于正常组织 (P <0 .0 1) ;在肿瘤组织中 ,两者的表达与患者的性别、年龄、病变部位、T分期无关 (P>0 .0 5 ) ,而与病理分化程度和颈淋巴结转移相关 (P<0 .0 5及 P<0 .0 1) ,在肿瘤组织中两者的表达存在相关性 (P <0 .0 5 )。结论 :VEGF和iNOS在喉鳞状细胞癌的生长、发展和转移中起重要作用 ,两者可能通过相互上调表达而密切相关 ,共同参与喉鳞状细胞癌的生长、发展和转移。

Objective:To investigate the mRNA expression of vascular endothelial growth factor(VEGF) and inducible nitric oxide synthase(iNOS) in laryngeal squamous carcinoma and its correlation with clinicopathologic parameters, to identify the interactions between the expression of VEGF and iNOS.Method:The mRNA expression of VEGF and iNOS in fresh laryngeal squamous carcinoma tissues and adjacent normal tissues from 40 patients were examined by reverse transcription-polymerase chain reaction.Result:The positive expression rate of VEGF and iNOS in tumor samples were significantly higher than that in normal tissues(P<0.01); in tumor samples, their expression were not associated with age, sex, tumor site and T stage(P>0.05); their expression were higher in moderately and poorly differentiated tumors than in well differentiated tumors(P<0.05);their expression were significantly higher in tumors with cervical lymph node metastasis than in tumor without cervical lymph node metastasis(P<0.01).There were correlation between the expression of VEGF and iNOS in tumor samples (P<0.05).Conclusion:VEGF and iNOS could up-regulate each other to promote the development and cervical lymph node metastasis of laryngeal squamous carcinoma.