绿衣枳实挥发油的GC-MS分析

Analysis of Essential Oil from Fructus Ponciri Trifoliatae Immaturi by GC-MS

目的建立气相色谱-质谱法对绿衣枳实挥发油进行成分分析。方法采用水蒸气蒸馏法从绿衣枳实中提取挥发油,利用GC-MS联用技术进行分离,所得的成分质谱数据经Wiley138和NBS75K.L质谱数据库检索并与有关标准图谱资料核对鉴定,从而确定出绿衣枳实挥发油中的化学成分,应用色谱峰面积归一化法测定各成分的相对含量。结果挥发油中共鉴定出21个化合物,占挥发油总量的99.04%。主要成分是:柠檬烯(55.68%)、β-月桂烯(21.96%)、β-蒎烯(5.17%)、顺式-石竹烯(4.61%)、β-罗勒烯(1.71%)。结论该方法可靠,可用于绿衣枳实挥发油的成分分析。

Objective To establish a GC-MS method for the analysis of the chemical components of essential oil from Fructus Ponciri Trifoliatae Immaturi.Methods The essential oil was extracted by steam distilyation ,then separated by capillary gas chromatography. The components from essential oil were identified by comparing with related standard chromatogram and their amount were determined by normalization method.Results Twenty-one components have been identified from Folium Fructus Ponciri Trifoliatae Immaturi and their amount accounted 99.04 %of total essential oil. The main components are limonene (55.68 %),β-myrcene(21.96 %),β-Pinene(5.17 %),cis-caryophyllene(4.61 %)and β-Ocimene (1.71 %).Conclusion This method is reliable and effective and can be applied to analyze the essential oil components extracted from Fructus Ponciri Trifoliatae Immaturi.