EAE大鼠中枢神经系统与外周免疫器官细胞凋亡的研究

A Study of Cell Apoptosis in the Central Nervous System and the Peripheral Lymphoid Organs in EAE Rats

目的 比较实验性自身免疫性脑脊髓炎 ( experimental autoimmune encephalomyelitis,EAE)大鼠中枢神经系统 ( CNS)与外周免疫器官中细胞的凋亡情况。方法 建立 EAE大鼠模型 ,于病程不同阶段取脊髓与外周免疫器官通过脱氧核糖核苷酸末端转移酶介导的缺口末端标记 ( TUNEL)技术检测细胞凋亡情况。结果 脊髓中细胞凋亡现象具有明显的时程特异性 ,凋亡细胞主要为淋巴细胞、巨噬细胞和小胶质细胞 ;脾和淋巴结中TUNEL阳性细胞数目均较少 ,且组内与组间差异均无显著性。结论 EAE外周免疫器官与

Objective To study the apoptotic lymphoid cells in the central nervous system (CNS) and the peripheral lymphoid organs in experimental autoimmune encephalomyelitis (EAE) rats. Methods Lymph nodes, spleens and lumbar spinal cords were removed form EAE rats at various stages of the course, and embeded with paraffin for cytochemical TUNEL detection. Results The number of TUNEL positive cells has an obvious course related character in the spinal cord in EAE, and the majority of TUNEL positive cells were T cells,macrophages and microglia judged by the morphological features. TUNEL positive cells could be found occasionally in the lymph nodes and spleens, while no significant difference was found between the two groups. Conclusions The various environmental conditions in CNS and peripheral lymphoid organs may affect the occurrence of apoptosis.