宫颈鳞状细胞癌HPV16/18、p53、p21表达及意义

Expression of HPV16/18、p53、p21 in squamous cell carcinoma of the cervix

目的 探讨人乳头状瘤病毒 (HPV) 16型、18型及 p5 3、p2 1基因蛋白的表达情况以及与宫颈癌的关系。 方法 应用免疫组化二步法检测 5 0例宫颈鳞癌、4 0例正常宫颈黏膜中HPV16、HPV18、p5 3、p2 1的表达。肿瘤组分 <6 0岁和≥ 6 0岁 2个年龄组 ,观察HPV感染情况。结果 宫颈鳞癌中HPV16、HPV18、p5 3、p2 1表达分别为 4 8%、2 0 %、5 4 %和 5 0 % ,而正常宫颈黏膜中的表达分别为 10 %、0、0、10 % ,两者经统计学比较差异有显著性 (P <0 0 1) ;<6 0岁组和≥ 6 0岁组HPV16阳性率分别为 84 2 %和 2 5 8% ,年青组明显高于年老组 ,经统计学比较差异有显著性 (P <0 0 1)。结论 (1)宫颈鳞癌的发生与HPV16、HPV18感染有密切关系 ,提示检测宫颈HPV16、HPV18感染情况对于宫颈鳞癌的随访和早期诊断有着重要的参考价值。 (2 )提示宫颈黏膜在HPV感染后 ,可能在p5 3、p2 1多种癌基因的共同作用导致宫颈鳞癌的发生发展。

Purpose To investigate the expression of human papilloma virus (HPV) types 16, 18 and p53, p21 protein, and to evaluate their correlation with human cervical carcinoma(CCa). Methods 50 cases of cervix squamous cell carcinoma were divided into two groups, <60 and ≥60 years old. Then Immunohistochemistry was used to detect HPV16, 18, p53 and p21 proteins in these carcinomas and 40 cases of normal cervixes. Results The positive rates of HPV16, 18 and p53 and p21 were 48%, 20%, 54%, 50% in CCa. The positive rates of them in normal cervixes were 10%, 0, 0, 10%. The differences in these two growps were significant (P<0.01). The positive of HPV16 in <60 group and ≥60 group were 84.2%, 25.8%. With a significant difference between them(P<0.01). Conclusions (1)HPV16 and 18 infections are closely related with CCa. The examine of HPV16 and 18 viruses could be useful indicators for early diagnosis of CCa. (2) This study suggested that abnormal expression of HPV,p53 and p21 proteins may be involed in Cca development.