摘要
微核实验是短期检测遗传毒性化学物的最常用的方法。以小鼠为实验材料,以MN的化合物(二氯化锰)作为受试物,进行了骨髓细胞微核率的检测,推断出二氯化锰是一种染色体诱变剂,对人及动物具有潜在的致突变和遗传损伤作用。通过对小鼠不同染毒次数、不同采样时间微核数的观察,表明多次染毒并于末次染毒后12h取样,微核观察计数的效果好。
Micronucleus test is often used to examine genotoxicity compound in a short time.Through injecting Manganese compound (MnCl_2) into rat's abdominal cavity and observing Micronucleus cell in polychromatic erythrocyte(MNPCE),we deduce MnCL_2 may be a chromosome mutagen,which has potential mutagenicity and genetic damage.In addition,comparing with the results of different injecting toxic times and different sampling time,we know that on condition that rats are injected toxin 5 times and marrow are sampled in 12 hours after the last injection,the effect of observing MNPCE is better than the others.
出处
《新疆农业科学》
CAS
CSCD
2004年第6期416-418,共3页
Xinjiang Agricultural Sciences
基金
新疆农业大学
