摘要
海岛棉的茎尖培养需要特定的适宜营养和激素条件,新海5号和K354在KT0.5mg/l和IAA0.2mg/l的MS培养基上适宜生长;新海16号在KT1.0mg/l和IAA2.0mg/l的MS培养基上生长分化良好。对比发现激素配合使用在一定浓度范围内有利于茎尖的生长分化。苗龄大小在一定程度上影响茎尖的生长分化进程和程度,3日、4日苗龄的茎尖生长分化速率明显高于2日、5日苗龄的茎尖,4日苗龄的茎尖分化程度又较3日苗龄的茎尖显著。
The culture of Gossypium barbadense from shoot apex tissues needed appropriate hormone condition. MS medium with KT 0.5 mg/l and IAA 0.2 mg/l was suitable for shoot apex tissues of 'No.5 Xinhai'and K354.Shoot apex tissues of 'No.16 Xinhai'differentiated well at MS medium containing KT 0.5 mg/l and IAA 0.1 mg/l. Results showed at range of hormone proportion was advantageous to growth of shoot apex tissues.To a certain extent,the age of seeding affected process of shoot apex tissues differentiation,and shoot apex tissues of the fourth day seeding are fit for growth and differentiation.
出处
《新疆农业科学》
CAS
CSCD
2004年第6期445-447,共3页
Xinjiang Agricultural Sciences
基金
新疆长绒棉品质改良基因工程项目
关键词
新疆
海岛棉
茎尖培养
激素
培养基
生长分化速率
Gossypium barbadense
shoot apex tissues culture
hormone
age of seeding