用直接凝集试验与间接荧光抗体试验检测弓形虫的比较研究

EVALUATION OF DIRECT AGGLUTINATION TEST ON TOXOPLASMA: COMPARED WITH INDIRECT FLUORESCENT ANTIBODY TEST

为了评价新建立的弓形虫直接凝集试验(DAT),对DAT和常规的间接荧光抗体试验(IFA)作了比较研究。用两种方法平行检测了41份商品免血清、76份人工感染的兔血清和271份人血清。定性和定量比较的结果显示:DAT与IFA有很高的符合率(93.4～95.9％);DAT较IFA敏感,前者的几何平均滴度高于后者,在检测感染免血清时,DAT远较IFA敏感。通过比较,作者认为DAT更适用于弓形虫感染的大规模筛选和血清流行病学研究。

Toxoplasma direct agglutination test (DAT) was evaluated by comparing it with the conventional indirect fluorescent antibody test (IFA). High agreements were qualitatively shown between DAT and IFA, 95.1％, 93.4％ and 95.9％ for sera of commercial rabbits, experimentally infected rabbits and human respectively. The geometric mean titers (GMT) of DAT were higher than those of IFA. The DAT titers were markedly high as compared with those of IFA on sera from experimentally infected rabbits. Because of its high sensitivity and specificity and the ease and low cost of its application compared to the IFA, the DAT is considered to be useful for mass screening and sero-epidemiological studies of Toxoplasma infection.