用基因表达谱芯片筛选老年性痴呆基因表达谱

Selection of Gene Expression Profile of Alzheimer's Disease by cDNA Microarray

[目的 ]筛选老年性痴呆 (AD)异常表达基因 ,探讨其在AD病生理机制中的作用。 [方法 ]利用小鼠 15 4 0 0点基因表达谱芯片 ,应用AD替代研究模型SAMP8,通过 10月龄SAMP8与同月龄的正常对照SAMR1比较和与同品系正常年轻 2月龄时的SAMP8比较 ,筛选出AD基因表达谱。 [结果 ]筛选到痴呆相关的差异表达基因 2 4 2个 ,衰老相关的差异表达基因 2 34个。在两组中同时出现的基因 ,即为筛选到既与痴呆又与衰老相关的差异表达基因 ,共110个。以上筛选到的差异表达基因均以下调表达为主 ,下调表达基因的数目几乎为上调的 2倍。 110中的 2 2个基因功能已知 :下调涉及突触囊泡释放 ,信号传导 ,细胞骨架 ,能量代谢 ;上调涉及细胞周期 ,离子通道 ,蛋白质合成 ,炎症反应等病生理功能。 [结论 ]本研究所得到的“老年性痴呆表达基因谱”能从众多研究线索中发现一些规律 ,为今后减少研究的盲目性提供依据。

Objective To select the abnormal expression genes of Alzheimer's Disease(AD) in Senescence Accelerated Mouse(SAM) and discuss their role in the pathogenesis of AD.[Methods]Using 15 400 mouse cDNA microarray the abnormal expression genes in AD were screened.[Results]Two hundred and forty two genes related to dementia were selected, 234 genes related to senile were selected. One hundred and ten genes were selected in both groups considered to be related to both senile and dementia. Most the selected genes were down-regulated,the number of them were almost two-fold than that of up-regulated. The gene function of 22 in 110 were known. The down-regulated genes were mostly related to the pathological functions in releasing of synaptic vesicle, signal transduction, cell skeleton, energy metabolism, etc. On the other hand, the up-regulated genes were mainly involved in the pathologic functions of cell cycle, ion channel, protein synthesis, inflammatory reaction, and so on.[Conclusion]“Gene expression profile of AD” may find some rules in many clues in researches of AD and provide some evidences to reduce the blindness in the late study.