丹酚酸B预适应对缺氧复氧损伤细胞热休克蛋白70mRNA表达的影响

Effect of Salvianolic Acid B Preconditioning on Expression of Heat Shock Protein 70mRNA in Cardiac Microvascular Endothelial Cells Injured by Hypoxia and Reoxygenation

[目的 ]探讨丹酚酸B(SalB)预适应的细胞保护机制是否与诱导热休克蛋白 70 (HSP70 )合成有关。[方法 ]在缺氧复氧损伤大鼠心脏微血管内皮细胞 (CMEC)后 ,采用RT -PCR法观察SalB预适应对细胞HSP70mR NA表达的影响。 [结果 ]HSP70mRNA在正常的CMEC有较低表达 ,缺氧刺激使其表达明显增强。SalB预适应促进HSP70mRNA表达增强 ,作用明显优于缺氧预适应 (HPC)。晚期预适应保护中 ,SalB预适应同样促使HSP70mRNA表达明显增强 ,与HPC具有增强表达的叠加效应。 [结论 ]SalB预适应通过诱导HSP70表达升高发挥细胞保护作用。

Objective To explore whether the mechanism of the cellular protection of salvisnolic acid B (Sal B) preconditioning is relevant with the synthesis of the heat shock protein 70 (HSP70). [Methods] Technique of RT-PCR was adopted ,and the impact of Sal B preconditioning on the expression of heat shock protein 70 (HSP70) mRNA in cardiac microvascular endothelial cells(CMEC) injured by hypoxia and reoxygenation was observed. [Results] HSP70 produced less expression in normal CMEC and its expression was increased significantly by hypoxia stimulating. Sal B preconditioning enhanced the expression of HSP70 mRNA and the effect was obviously superior to that of hypoxic preconditioning (HPC). During the delayed preconditioning protection, Sal B also could increase the HSP70 mRNA expression and enhance the effect of HPC. [Conclusion] Sal B preconditioning produces a protective effect on cells by inducing the expression of HSP70 .