孕妇乙型肝炎病毒DNA含量与母乳感染的关系

The correlation between DNA content of hepatitis Bvirus in maternal serum and infection to breask milk

【目的】观察孕妇乙型肝炎病毒DNA含量与母乳感染的关系。【方法】采用ELISA法检测孕妇产前血清与产后 2～ 5d乳汁乙型肝炎病毒标志物 (HBVM ) ,对HBVM阳性的孕妇用乙型肝炎病毒核酸扩增酶联免疫法 (HBV PCR ELISA)检测产前 1～ 2d或产后 1～ 2d血清HBV DNA含量。【结果】HBV感染孕妇 80例 ,其中单阳 5 4例 ,双阳 2 6例。单阳组有 4 2例、双阳组有 2 1例检测血清HBV DNA含量 ,结果单阳组HBV DNA阳性 7例 ,双阳组 18例 ,经 χ2 检验P <0 0 1,差异有显著性 ;单阳组有 4 9例检测乳汁HBVM ,阳性 13例 ,双阳组 17例HBVM阳性 10例 ,经 χ2 检验P <0 0 5 ,差异有显著性。【结论】孕妇HBV DNA阳性时 ,其乳汁HBV感染的危险性增加。

To observe the correlation between DNA content of hepatitis B virus (HBV) in mat ernal serum and infection to breast milk.ELISA was used to test hepatitis B virus marker(HBVM) in maternal serum before delivery and in breast milk 2～5 days after delivery.Serum HBV-DNA content was tested by HBV-PCR-ELISA within 1～2 days before or after delivery for HBVM-positive pregnant women. It was shown that 80 cases were HBV-infected,among which 54 cases were HBsAg po s itive and 26 cases were HBsAg and HBeAg positive.The serum HBV-DNA content was t ested in 42 HBsAg-positive and 21 HBsAg-and HBeAg-positive pregnant woment,an d 7 cases in HBsAg-positive group and 18 cases in HBsAg and HBeAg positive grou p were positive.There was a significant difference between the two groups by chi -square test(P<0.01).The breast milk was tested for HBVM,and 13 of 49 in HBsAg-pos itive group and 10 of 17 in HBsAg-and HBeAg-positive group were positive.There was a significant difference between the two groups by chi-square test (P< 0.05).[Conclusion]The risk of infection to breask milk is higher when pr egnant woman is HBV-DNA positive.