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Establishment and Preliminary Application of Two dimensional Electrophoresis and Mass Spectrometry in Ovary Study 被引量:1

Establishment and Preliminary Application of Two dimensional Electrophoresis and Mass Spectrometry in Ovary Study
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摘要 Objective To apply two-dimensional electrophoresis and mass spectrometry in the ovary proteome researchMethods Protein extractions from mouse ovaries were run in IPGphor isoelectric focus system with 11 cm and 24 cm IPG strips respectively (pH 3~10, 0.3 mm thick), then the protein spots were identified by mass spectrometry.Results The ovary protein exactions separated by two-dimensional electrophoresis have got high resolution, and identifing protein by mass spectrometry was highly efficient and facilitly. These two techniques should facilitate further investigation of female reproduction proteome research.Conclusion These two rapid high resolutions and efficient techniques have a variety of applications foreground in female reproduction proteome pattern research. Objective To apply two-dimensional electrophoresis and mass spectrometry in the ovary proteome researchMethods Protein extractions from mouse ovaries were run in IPGphor isoelectric focus system with 11 cm and 24 cm IPG strips respectively (pH 3~10, 0.3 mm thick), then the protein spots were identified by mass spectrometry.Results The ovary protein exactions separated by two-dimensional electrophoresis have got high resolution, and identifing protein by mass spectrometry was highly efficient and facilitly. These two techniques should facilitate further investigation of female reproduction proteome research.Conclusion These two rapid high resolutions and efficient techniques have a variety of applications foreground in female reproduction proteome pattern research.
出处 《Journal of Reproduction and Contraception》 CAS 2003年第4期209-216,共8页 生殖与避孕(英文版)
关键词 PROTEOME two-dimensional electrophoresis matrix assisted laser desorption/ ionization time of flight mass spectrometry (MALDITOF MS) OVARY proteome two-dimensional electrophoresis matrix assisted laser desorption/ ionization time of flight mass spectrometry (MALDITOF MS) ovary
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