发根农杆菌促进黑荆树切根幼苗生根

Agrobacterium rhizogenes Riker Promotes the Rooting of Root-cut Seedling of Acacia mearnsii De Wild

利用发根农杆菌A_4、9402菌株接种黑荆树切根幼苗,在MS培养基上促进其生根,生根率达96％～98％,平均每株苗生根17～19条,且促进幼苗高生长。冠瘿碱检测证明新生根是Ri T-DNA转化根。Ri T-DNA转化细胞生根效应与植物激素有相关性。

Root-cut seedling of Acacia mearnsii were inoculated with Agr-obactfrium rhizogences A4, 9402, and rooting was promoted on MS medium. The rooting ratio was 96%~98%, and the average number of new roots per seedling was 17~19, but the root-cut seedling treated with medium alone didn't root. Opine assay indicated that there was stable maintenance of T-DNA in these new roots. There was some relationship between auxin and A. rhizogencs T-DNA in the induction of rooting.