锗载体导入人参培养细胞的研究

Study on Introduction of Germanium into Panax ginseng Cultured Cells via Chemical Vectors

野山参在数十年的生长期中富集了大量的锗,药效显著。人参培养细胞吸收和积累的锗量很少,约8～17ppm。制备了对细胞无毒害的载体化合物VA、VB和VC,利用载体把锗导入人参培养细胞,培养周期28d,载体VA、VB导入的锗量分别达到2978ppm和483ppm。人参富集锗是成熟细胞的功能,在细胞培养的对数期末载体供锗是合理的。供锗培养不影响培养细胞人参皂甙的生物合成和积累。

A great quantity of germanium was abounded in wild Panax ginseng during its growing period for decades, and its medical effects were very strong. The content of germanium absorbed and accumulated in Panax ginseng cultured cells was about 8~17 ppm. Chemical vector compounds VA, VB, and VC of free toxicity for cells were prepared, and germanium was introduced into Panax ginseng cultured cells through using these vectors. During a growth period of 28 days, the quantity of germanium introduced via VA, VB, or VC attained upto 2978, 286, and 483 ppm respectively. Abound of germanium in Panax ginseng was a function of mature cells. Su-peraddition of germanium via vectors was reasonable at the end of logarithm period. Superaddition of germanium in culture had no influence on biosynthesis and accumulation of Panax ginseng saponin.