11-去氢-血栓烷B_2和11-β-前列腺素F_(2α)在糖尿病病人中变化的初探

An Initial Study of the Changes of 11-DH-TXB2 and 11-β-PGF2α Plasma Level in Patients with Diabetes Mellitus.

目的:观察糖尿病（DM）病人血小板活化状态及血管内皮细胞损伤的情况。方法:采用酶免疫（EIA）竞争法分别对21名正常健康人和50例2型糖尿病（non-insulin dependent diabetes mellitus,NIDDM）病人的血浆11-去氢-血栓烷B（11-dehydro-thromboxane B2,11-DH-TXB2）及11-β-前列腺素F2α（11-β-prostaglandin F2α）的浓度进行检测,分组进行对照分析。结果:DM病人与正常对照组比较,其血浆11-DH-TXB2浓度显著增高（P<0.01）,按临床表现将病人分组后,各组间也有不同程度的差异,其中有胰岛素抵抗病人的11-DH-TXB2浓度较无胰岛素抵抗病人有明显升高（P<0.01）。而测得的11-β-PGD2α血浆浓度在正常对照组与病人各组间无统计学差异。结论:DM病人体内血小板高度活化,且与DM合并胰岛素抵抗及血管病变的发生有关;11-DH-TXB2可作为检测DM病人体内血小板活化状态的可靠指标;11-β-PGF2α血浆浓度在DM病人体内受多方面因素影响无明显改变。

Objective: To investigate the status of platelet activation and the injury of vascular endothelium in N1DDM patients. Methods: The plasma level of 11-DH-TXB2αand 11-β-PGF2α were detected and analysed in 21 health controls and 50 patients by the method of competitive EIA. Results: The plasma level of 11-DH-TXB2 patients with NIDDM was significantly increased compared with the normal controls. It was divided into groups according to clinical manifestations and there were various plasma levels of 11-DH-TXB2 in different groups. Especially, the patients with insulin resistance were found to have higher level of plasma 11-DH-TXB2. Plasma levels of 11-β-PGF2α were not significant in each groups including the controls. Conclusions: The platelet was highly activated in the patients with NIDDM. High plasma level of 11-DH-TXB2 and massive platelets activation were closely related to NIDDM patients with insulin resistance and injury of vascular endothelium. Plasma level of 11-DH-TXB2 was detected as a reliable indicator of evaluating the status of platelet activation in patients with DM.