利用生物信息学方法预测HAb18G/CD147拮抗肽的性质和功能

Analyzing HAb18G/CD147 antagonistic peptides using bioinformatics

目的 :利用生物信息学方法对HAb1 8G/CD1 4 7拮抗肽 (AP 1～ 9)进行理化性质的预测及相似性的比较 ,为拮抗肽的结构与功能研究提供预测和帮助 .方法 :应用ExPASy数据库分析短肽的Mr、等电点、疏水性等理化性质 .利用Blastp,PDB_ISL和SCOP等数据库对HAb1 8G/CD1 4 7拮抗肽进行相似性比较 .结果 :经ExPASy数据库分析 9条拮抗肽中有 6条呈碱性 ,且大部分呈亲水性 ,半衰期由 1 .3～ 30 .0h不等 .Blastp分析AP 2与血管紧张素转化酶Ⅱ的活性中心部分序列相似 .PDB_ISL分析AP 3,9与NADPH氧化酶部分序列相似 .SCOP分析AP 2与Ras结合蛋白 (RBD) ,CAD结构域及金属蛋白酶催化结构域相似 ;AP 7与环亲和素及微管蛋白C 末端结构域相似 .结论

AIM: To analyze and predict the physical and chemical characteristics of HAb18G/CD147 antagonistic peptides (APs), to compare its similarity with other known proteins and to provide some evidence for further research on antagonistic peptides’ structures and functions. METHODS: Integrated bioinformatics database ExPASy was used to analyze APs’ molecular weight ( M r), isoelectric point (pI), hydrophobicity and half life. Blastp, PDB_ISL and SCOP database were used to analyze the similarity with other known proteins. RESULTS: It was found that 6 of the 9 APs were rich in basic amino acids, most of which were hydrophilic and their half life was 1.3-30.0 h. Similar sequences were found between AP 2 and angiotensin converting enzymeⅡ (ACEⅡ) by Blastp, between AP 3, AP 9 and NADPH oxidase by PDB ISL, between AP 2 and Ras binding protein (RBD), CAD domain and metalloproteinases catalytic domain, and between AP 7 and cyclophilin and tubulin C terminal domain by SCOP. CONCLUSION: Bioinformatical methods are helpful in the study of polypeptide and protein structure and function prediction as well as drug design.