一管法测定补体C1溶血活性

THE FIRST COMPONENT OF HUMAN COMPLEMENT

本文用400.0g/L 聚乙二醇沉淀正常人新鲜血清,制备出C1缺损血清(C_1D),用一管法测定C1溶血活性。经免疫双向扩散等实验证实C_1D 血清仅去除C1,补体其它成份基本不受影响,因而能特异性测定C1溶血活性。经方阵滴定,待检血清与C_1D 最适稀释度为1∶20;本法批内CV=5.5%;与多管法测定结果比较,r=0.9155,p<0.01。用一管法检测慢性活动性肝炎等患者血清C1溶血活性,其结果明显低于正常人。本法简便,快速,适于一般实验室应用。

The single tube assay for the hemolytic activity of the first component of hu-man comlement(Cl)with Cl deficient scrum(ClD)was reported.The complement compo-nents,except Cl,remained in the ClD and so ClD could detect Cl hemolytic activity specially.In the assay,the best dilution of serum and ClD were 1:20 all;CV=5.50%.The results ofthe single tube procedures related well with these of the multiple tube procedures(r=0.9155,P<0.01).Cl hemolytic activity was lower markedly in the patients with chronic activehepatitis,nephritis and conncctive tissue diseases than in the healthy controls,which indi-cated Cl activation apparently in these patients.It is considered that this method is simple,stable and reliable,and may be applicable to general laboratory.