肝硬变组织内HBV DNA和HBsAg与肝细胞不典型增生关系的研究

A Study of Relationship Between HBV DNA and HBsAg and Liver Cell Dysplasia in Cirrhotic Liver

应用原位杂交与免疫组化PAP法相结合的双标记技术,对51例肝硬变和9例癌周肝硬变组织内乙肝病毒DNA(HBV DNA)和HBSAg与肝细胞不典型增生(LCD)的关系进行了探讨。结果显示,60例肝硬变组织中,16例具有LCD和44例无LCD的肝硬变组织内HBV DNA的阳性检出率分别为75.0%和40.9%,HBV DNA和HBsAg双标阳性的检出率分别为75.0%和36.4%,均以具有LCD的肝硬变组织为高(P<0.05)。结果说明,HBV的感染以及HBV DNA在肝硬变组织内的持续存在、活跃复制和表达与肝硬变组织发生LCD和癌变关系密切。

The relationship between hepatitis B virus DNA(HBV DNA)and HBsAg and liver celldysplasia(LCD)was studied in the tissues of 51 liver cirrhosis and 9 paratumor cirrhotic liver by thedouble labelling technique of in situ hybridization and immunohistochemical PAP method.The resultsshowed that the positive rates of HBV DNA in 16 cirrhotic tissues with LCD and 44 cirrhotic tissueswithout LCD were 75.0% and 40.9%,respectively,and the positive rates of both HBV DNA andHBsAg in those tissues were 75.0% and 36.4%,respectively.The positive rates of the both arehigher in the tissues with LCD than in the tissues without LCD(P<0.01).The results indicate thatthe infection of HBV,and persistent existence and active replication and expression of HBV DNA incirrho-tic tissues are closely related to the occurrence of LCD and hepatocellular carcinoma.