摘要
To investigate the relation between acupuncture stimulation and cellular immunity and the relation between the expression of cfosmRNA and ppENKmRNA or MEKIR and DynIR, the effect of acupuncture on the circulating lymphocyte cfosmRNA, ppENKmRNA, MEKIR and DynIR was studied. 20 BALB/C mice were randomly divided into 2 groups: a) acupuncture group, treated with 5 Hz electroacupuncture(EA); b) control group, treated with restraint but no EA. The mouse pain threshold was detected by K+ ionophoresis before and after EA or restraining. The mouse lymphocyte cfosmRNA and ppENKmRNA were demonstrated by in situ hybridization and RNA dot blot. The mouse lymphocyte MEKIR and DynIR were detected by immunohistochemistry and protein dot blot. The dot blot signals were scanned for statistical analysis. The results showed that all the signals offosmRNA, ppENKmRNA, MEKIR and DynIR were localized in the lymphocyte cytoplasma and stronger in the acupuncture group than that in the control group(P<0.05). All of the signals were positively correlated with the analgesic effect in the EA group(P<0.05-0.01). Besides, there was a positive correlation on alteration between cfosmRNA and ppENKmRNA or between cfosmRNA and DynIR(P<0.05), suggesting that cFos may be involved in ppE or ppD gene transcription in the mouse lymphocyte.
To investigate the relation between acupuncture stimulation and cellular immunity and the relation between the expression of cfosmRNA and ppENKmRNA or MEKIR and DynIR, the effect of acupuncture on the circulating lymphocyte cfosmRNA, ppENKmRNA, MEKIR and DynIR was studied. 20 BALB/C mice were randomly divided into 2 groups: a) acupuncture group, treated with 5 Hz electroacupuncture(EA); b) control group, treated with restraint but no EA. The mouse pain threshold was detected by K+ ionophoresis before and after EA or restraining. The mouse lymphocyte cfosmRNA and ppENKmRNA were demonstrated by in situ hybridization and RNA dot blot. The mouse lymphocyte MEKIR and DynIR were detected by immunohistochemistry and protein dot blot. The dot blot signals were scanned for statistical analysis. The results showed that all the signals offosmRNA, ppENKmRNA, MEKIR and DynIR were localized in the lymphocyte cytoplasma and stronger in the acupuncture group than that in the control group(P<0.05). All of the signals were positively correlated with the analgesic effect in the EA group(P<0.05-0.01). Besides, there was a positive correlation on alteration between cfosmRNA and ppENKmRNA or between cfosmRNA and DynIR(P<0.05), suggesting that cFos may be involved in ppE or ppD gene transcription in the mouse lymphocyte.
