E试验(E test)检测人型支原体对四环素的药物敏感性初步评价

Examination of the Susccptabilities of Mycoplasma Hominis to Tetracycline by E test;A Prima-E the; A Primary Evaiuation

目的:评价 E test用于检测人型支原体(Mh)对四环素敏感性的可行性。方法:同时应用肉汤稀释法和 E test检测了 20株 Mh对四环素的药物敏感性,比较两种方法抑制 Mh生长的最低抗生素浓度(MIC)值。结果:和肉汤稀释法MIC相比。E test有 70%(14/20)结果相差在±1个稀释度内,两种方法±2个稀释度内的符合率为 100%(20/20)。结论:E test操作简单,使用方便,结果客观,是检测 Mh对四环素药物敏感性可行方法之一。

To evaluate the passibility of E test for susceptibility testing of Mh to tetracy-cline. Methods: Both microdilution method and E test were used simultaneously to determine the MIC of20 clinical isolates of Mh, and the results were explored. Results: The agreement between two methodswas ± one dilution and ± two dilutions in 70% and 100% of cases. Conclusion: Compared with theboth microdilution method, E test showed the simplicity, convenience and objectivity and it can beadopted for susceptibility testing of Mh to tetracycline.