高效液相色谱法测定红葡萄酒中白藜芦醇的四种异构体

Analysis for Four Isomers of Resveratrol in Red Wine by High Performance Liquid Chromatography

将红葡萄酒用 β 葡萄糖苷酶水解处理 ,使其中的白藜芦醇甙转换成白藜芦醇。采用高效液相色谱法测定水解前后顺、反式白藜芦醇含量的变化 ,从而计算出葡萄酒中白藜芦醇的 4种异构体的含量。色谱柱为SymmetryC18柱 (3 9mmi.d .× 15 0mm) ,流动相为乙醇 0 0 5 %乙酸水溶液 (体积比为 2 3∶77) ,紫外检测波长为 30 6nm。实验结果表明 :白藜芦醇的 4种异构体在其质量浓度为 0 2～ 5 0 .0mg/L时均有良好的线性关系 (r >0 999) ,白藜芦醇及其甙的顺、反异构体的最小检出量分别为 :0 81,1 2 0 ,0 4 0和 0 4 6ng ,白藜芦醇回收率大于 98% ,相对标准偏差 (RSD)为 0 5 %～ 8 5 %。 11种被测干红葡萄酒中白藜芦醇总醇含量为 0 84～ 7 33mg/L,3种被测葡萄汁中白藜芦醇总醇含量为 0 12～ 6 .0 0mg/L。该方法操作简单 ,精密度高 ,重复性好 ,可用于葡萄酒 (汁 )中白藜芦醇及其甙的顺、反异构体的测定。

Red wine was treated by hydrolysis with β-glucosidase, so the piceids in it were converted to resveratrols. The contents of cis- and trans-resveratrols before and after hydrolysis were determined by high performance liquid chromatography, thereby the contents of the four isomers of resveratrol in the red wine can be calculated. The analysis was performed on a Symmetry C_(18) column (3.9 mm i.d.×150 mm) with a mixture of ethanol and 0.05% acetic acid solution (23∶77, v/v) as the mobile phase at a flow rate of 1.0 mL/min and with UV detection at 306 nm. Results show that there was a good linear relationship (r>0.999) for all the four (isomers) of resveratrol in the range of 0.2-50.0 mg/L. The detection limits of cis- and trans-resveratrol and the corresponding piceid (isomers) were 0.81, 1.20, 0.40 and 0.46 ng, respectively. The recovery of resveratrol was more than 98%. Relative standard deviations ((RSDs)) (n=5) were from 0.5% to 8.5%. The ranges of concentration of total resveratrol were 0.84-7.33 (mg/L) in eleven dry red wines and 0.12-(6.00 mg/L) in three grape juices. The method is convenient, and there are good precision and repeatability. The method is applicable for the (analysis) of four isomers of resveratrol in red wines and grape juices.