大鼠弥散性脑损伤hsp70mRNA表达的研究

A Study on Expression of hsp70 mRNA in the Early Stage of Diffuse Brain Injury in Rats

目的 寻找脑损伤早期诊断指标。方法 复制大鼠弥漫性脑损伤模型 ,用原位杂交和图象分析技术检测不同损伤时间的大鼠脑干、丘脑、大脑皮层等部位神经元和神经胶质细胞中 hsp70 m RNA表达情况。结果 发现不同损伤时间 ,hsp70 m RNA表达的强弱不同 ;在损伤 5 m in后即可在脑干、丘脑、皮质等部位检测到 hsp70 m R-NA,6 h达高峰 ,12 h后减弱。结论 hsp70 m RNA可以作为早期脑弥散性损伤的指标 ,并可用于生前伤与死后伤的判定。

Objective To evaluate the changes of hsp70 mRNA in neuron and glia of rat suffering early diffuse brain injury. Methods SD rats were used in the replication of the animal pattern of diffuse brain injury. In situ hybridization technique and image analysis technique were applied in detecting the hsp70 mRNA in the rat's cerebral cortex, thalamus and brain stem. Results The signal intensity of hsp70 mRNA could be detected in 5 minutes after brain injury; subsequently, it attained to the summit at 6 hours and declined at 12 hours after brain injury. Conclusion The above data suggest that hsp70 mRNA is useful for the diagnosis of early diffuse brain injury and for distinguishing the postmortern injury from antemortem injury.