用质谱法研究烟草花叶病毒外壳蛋白

This paper introduced a rapid, sensitive, and exact method to identify the different strains of plant virus. One strain of tobacco mosaic virus(TMV)was isolated from tobacco plants, its coat protein (TMV-CP) was analyzed by MALDI-TOFMS, the molecular weight and peptide mass spectra of trypsin enzymolysis were used to identify virus strain. Compared it with other TMV strains in the internet PDBdatabase, the amino acid sequence of this stain was rather similar to one of 12 strains. The results shown mass spectrometry was suitable for identification plant virus rather than SDS-PAGE method.