同位素质谱法检测尿中雄烯二酮代谢物

A method was established for measuring the isotope ratios of ^13C to ^12C of the metabolites of androstenedione in urine. The source of these metabolites could be distinguished. The metabolism time of the steroids could be detected with this method. Preliminary separation of endogenous anabolic androgenic steroids could be achieved using enzymolysis, HPLC. The source of the metabolites of androstenedione could be detected byt heirδvalues with gas chromatography-combustion-isotope ratio mass spectrometry. The results showed that theδvalues of some metabolites of androstenedione reduced after the administration of androstenedione preparation. In these cases the data hinted that exogenous anabolic androgenic steroids were administrated. The source of metabolites of androstenedione in urine could be detected by measuring theirδvalues with this method in some period.