K88-ST融合基因的克隆及真核表达载体的构建

Constructiojn of the Recombinant Plasmid pCAMBIA-K-ST

利用DNA重组技术,将编码K88-ST融合基因的序列片段克隆到植物表达载体pCAMBIA1302中,成功的构建了植物重组表达质粒pCAMBIA-K88-ST。并将其转化农杆菌EHA105,为K88-ST基因的进一步研究奠定基础。

The plant recombinant expression plasmid pCAMBIA-K-ST has been successfully constructed by cloning the gene of K88-ST into the plant expression vector pCAMBIA1302. The molecular biological technique such as single-enzyme-insertion was also used in this research. The plant binary expression vector of K88-ST was transformed into A grobacterium EHA 105. It provided a reliable basis for the further study on K88-ST.